Running Head:
نویسندگان
چکیده
We report a gene discovery system for poplar trees based on gene and enhancer traps. Gene and enhancer trap vectors carrying the GUS reporter gene were inserted at random into the poplar genome, where they reveal the expression pattern of genes at or near the insertion sites. Because GUS expression phenotypes are dominant and are scored in primary transformants, this system does not require rounds of sexual recombination, a typical barrier to developmental genetic approaches in trees. Geneand enhancer-trap lines defining genes expressed during primary and secondary vascular development were identified and characterized. Collectively, the vascular gene expression patterns revealed that approximately 40% of genes expressed in leaves were expressed exclusively in the veins, indicating that a large set of genes is required for vascular development and function. Also, significant overlap was found between the sets of genes responsible for development and function of secondary vascular tissues of stems and primary vascular tissues in other organs of the plant, likely reflecting the common evolutionary origin of these tissues. Chromosomal DNA flanking insertion sites was amplified by TAIL PCR and sequenced, and used to identify insertion sites by reference to the nascent Populus trichocarpa genome sequence. Extension of the system was demonstrated through isolation of full-length cDNAs for five genes of interest, including a new class of vascular-expressed gene tagged by enhancer trap line cET-1-pop1-145. Gene and enhancer traps provide a new resource that allows plant biologists to directly reference the poplar genome sequence and identify novel genes of interest in forest biology. 5
منابع مشابه
The timing pattern of selected muscles in male children with forward head posture compared to healthy control ones during running
Objective: The aim of this study was to evaluate the muscular activity timing during running in male children with forward head posture compared to healthy control ones. methods: 12 healthy male children with forward head posture (age: 11.7±1.4 years; height: 149.7±6.2 cm; Mass: 38.0±4.7 kg) and 16 healthy male children (age: 11.8±1.5 years; height: 148.2±6.6 cm; mass: 39.6±0.4 kg) were volunt...
متن کاملVarying Definitions of Online Communication and Their Effects on Relationship Research
Contact: [email protected] The running head cannot exceed 50 characters, including spaces and punctuation. The running head’s title should be in capital letters. The running head should be flush left, and page numbers should be flush right. On the title page, the running head should include the words “Running head.” For pages following the title page, repeat the running head in all caps without...
متن کاملAdolescent Depression 1 Running Head: ADOLESCENT DEPRESSION Adolescent Depression and Attachment
Heading (on all pages): running head plus page number Running head (a shortened version of the title is defined on the title page and used in the heading of your paper. Title, name and affiliation are centered. Heading is right justified. Running head is left justified.
متن کاملRunning Head: Affect regulation during learning 1 Affect Regulation during Learning: The Enhancing Effect of Cognitive Reappraisal Running Head: Affect regulation during learning
متن کامل
Running head: ALIBI TIMING AND ALIBI WITNESS MOTIVATION The effect of evidence timing and witness motivation upon juror evaluations of alibi witnesses and defendants Running head: ALIBI TIMING AND ALIBI WITNESS MOTIVATION
متن کامل
Head and Tibial Acceleration as a Function of Stride Frequency and Visual Feedback during Running
Individuals regulate the transmission of shock to the head during running at different stride frequencies although the consequences of this on head-gaze stability remain unclear. The purpose of this study was to examine if providing individuals with visual feedback of their head-gaze orientation impacts tibial and head accelerations, shock attenuation and head-gaze motion during preferred speed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2004